*Dox: Doxycycline is used in "Tet-on" and "Tet-off" tetracycline-controlled transcriptional activation to regulate transgene expression in organisms and cell cultures.
*tau- LacZ (LAC): Marker genes to trace neuronal activity during learning (c-Fos in this paper)
*Zif/Egr (ZIF): An immediate early gene; as a neuronal activity indicator during retrieval
*Teto: A promoter
A transgenic mouse, TetTag: tTA (tetracycline transactivator system) linked to the c-fos promoter.
Dox was used to preserve the c-Fos+ cells activity during the FC training until the retrieval, since they cut off the DOX to visualize those activated cells- as well as- capturing the ZIF+ cells shortly after the test.
12% of neurons tagged with LAC were
reactivated during retrieval.
The non-reactivated cells were not specific
to the CS-US training. They think the reactivated neurons could be the possible component of a stable engram or memory trace for conditioned fear.
There was no correlation between the estimated
freezing during tone retrieval and the number
of reactivated neurons in the BLA.
However, the number of reactivated neurons in
the lateral amygdala (LA) correlated with the
estimated freezing during tone retrieval but did
not correlate with the freezing during context
retrieval
12% of neurons tagged with LAC were
reactivated during retrieval.
The non-reactivated cells were not specific
to the CS-US training. They think the reactivated neurons could be the possible component of a stable engram or memory trace for conditioned fear.
There was no correlation between the estimated
freezing during tone retrieval and the number
of reactivated neurons in the BLA.
However, the number of reactivated neurons in
the lateral amygdala (LA) correlated with the
estimated freezing during tone retrieval but did
not correlate with the freezing during context
retrieval 